Flow cytometry is a widely used technology for simultaneous detection of several biomarkers at a single cell level.  This quasi-quantitative approach permits the investigator to identify heterogeneity in a sample and make connections between related and unrelated cells. However, the use of flow cytometry has been largely limited to analysis of intra and extracellular proteins […]

Regulatory, or suppressor, T cells (Treg) are a subpopulation of CD4+ T lymphocytes which are defined by the expression of forkhead transcription factor (FoxP3). All T cells come from progenitor cells in bone marrow, with differentiation happening in the thymus. Treg cells are not mature until a couple days later than effector T cells, which […]

For typical flow cytometry assays, specimens are processed separately with the antibody panels to enumerate lymphocyte subsets of interest, yielding a sample by sample processing for each individual being tested. Although several hundred samples can be processed using this approach, what if there were methods to optimize efficiency of the flow cytometry testing, depending on […]