Since the first flow cytometers became commercially available 40+ years ago, the field of flow cytometry has grown in leaps and bounds. The earliest flow cytometers could measure 1-2 cellular parameters, while today, we take for granted that flow cytometers allow us routinely measure up to 20+ fluorescent parameters simultaneously. Modern flow cytometers are also […]
Silent but Deadly, and Beneficial? – Natural Killer Cells Natural Killer (NK) cells play a major role in targeting and killing both tumor cells and virally infected cells in the body. Although they play a very similar role to cytotoxic T cells, these cells comprise an important component of the host’s innate immune system. Most […]
Flow Cytometry is considered a high complexity analysis technique that is routinely used during the drug development process to analyze tumor specimens. As a result, the need for a laboratory with highly trained individuals is essential. Pharmaceutical and biotech companies should have the flexibility to choose the most qualified laboratory to perform their testing, even […]
Allergies are one of the fastest growing maladies affecting first world countries. In fact, before 1980 peanut allergies, now so commonly and dangerously found in children, were virtually unheard of in both medical journals and the media. In the USA alone, the CDC reports that between 1997 and 2008, food allergies in children have risen […]
In pharmaceutical drug development, quality is critical. The FDA relies on quality driven documents to make decisions on the safety and effectiveness of regulated products and the approval of those products. While Quality Assurance (QA) is a clear regulatory requirement in some instances (i.e., studies required to be compliant with Good Laboratory Practices), it can […]
If you are looking to incorporate flow cytometry into your study or clinical trial, ensuring that precise and uniform data is generated by contract flow labs can seem difficult, or even overwhelming. In fact, a study conducted by Maecker et al. 2005 revealed that in a protocol conducted across 15 different laboratories, the inter-laboratory coefficient […]
Staining with intracellular antibodies has been a vital way to study the cytoplasmic antigens. In order to introduce antibodies to the interior of the cell there are many buffers that can be used to create openings in the cell’s membrane. This process is called permeabilization (perm). Without the cell membrane being permeabilized, intracellular markers cannot […]
Regulatory, or suppressor, T cells (Treg) are a subpopulation of CD4+ T lymphocytes which are defined by the expression of forkhead transcription factor (FoxP3). All T cells come from progenitor cells in bone marrow, with differentiation happening in the thymus. Treg cells are not mature until a couple days later than effector T cells, which […]
Controls are a vital part of any rigorous scientific process. Any experiment worth its peer-reviewed credentials requires controls to verify these data are valid, and that variables outside of the parameter of interest are not confounding the results in any way. In the rapidly-expanding field of flow cytometry, it is no different. However, finding the […]
For typical flow cytometry assays, specimens are processed separately with the antibody panels to enumerate lymphocyte subsets of interest, yielding a sample by sample processing for each individual being tested. Although several hundred samples can be processed using this approach, what if there were methods to optimize efficiency of the flow cytometry testing, depending on […]