The separation of Peripheral Blood Mononuclear Cells (PBMC) may be required for certain applications in flow cytometry. At FCSL we can provide support for PBMC separation as required for your specific flow cytometry assay. Please inquire to start the scientific discussion.
PBMCs must be separated from whole blood. At Flow Contract Site Laboratory (FCSL), we -use density gradient separation for most of our PBMC preparations.
As shown in Figure 1, each cell population exhibits a unique migration pattern through the medium and each layer contains specific blood components. The cells are isolated by extracting the respective layer. Isolated PBMCs are washed and then evaluated for cell viability and cell count -using the Guava PCA (Personal Cell Analysis) as shown in Figure 2 and 3.
Viable PBMCs are cryopreserved in freezing media. Our laboratory has both liquid nitrogen and -80° freezers for short term storage until the specific flow cytometry assay can be performed.
At FCSL we have extensive experience in PBMC isolation and cryopreservation. We can help your next PBMC isolation in support of flow cytometry assays with ensuring high cell viability and recovery, timely, standardized and quality results. Please inquire to start the scientific discussion.