Conventional Flow Cytometry has been limited in the use of fluorophores due primarily to spectral overlap of emission signals. Spectral Flow Cytometry captures the full emission spectrum of the fluorophores, creating Spectral Signatures for each fluorophore and significantly lessening the spectral overlap problem enabling 40+ marker analysis while maintaining high signal resolution. Conventional Flow Cytometers […]

Silent but Deadly, and Beneficial? – Natural Killer Cells Natural Killer (NK) cells play a major role in targeting and killing both tumor cells and virally infected cells in the body. Although they play a very similar role to cytotoxic T cells, these cells comprise an important component of the host’s innate immune system.  Most […]

Staining with intracellular antibodies has been a vital way to study the cytoplasmic antigens. In order to introduce antibodies to the interior of the cell there are many buffers that can be used to create openings in the cell’s membrane. This process is called permeabilization (perm). Without the cell membrane being permeabilized, intracellular markers cannot […]